High heat = longer, thinner tips; Low heat = shorter, thicker tips. Adjustable gravity force (Light/Heavy types).
The PC-10 is a vertical puller that utilizes gravity to shape glass capillaries. Its design focuses on stability and reproducibility:
More weight = sharper tips; Fewer weights = parallel, longer shanks. Sets the distance for the first and second pulls. narishige pc10 manual new
According to the Narishige FAQ , a common starting point for a firm tip involves: 65 Heater Level (Second Pull): 60 Weights: 4 (Two light, two heavy). 4. Maintenance and Troubleshooting Setting Values in the PC-10 Puller - NARISHIGE WEB NEWS
Controls the overall length and "shoulder" shape of the pipette. High heat = longer, thinner tips; Low heat
Shows heater levels and helps track the status of the output voltage during the process. 2. Operating Modes: Single vs. Two-Stage Pulling
The process stops after an initial "softening" pull and then performs a second, more intense pull. This is essential for producing firm microelectrodes with the sharp tips (0.1µm to several µm) required for patch clamp experiments. 3. Setting Your Parameters Its design focuses on stability and reproducibility: More
Minimizes output voltage variations to ensure the heater reaches the exact same intensity every time.
Mastering the Narishige PC-10: A Comprehensive Guide to the Dual-Stage Pipette Puller